Human Histo-biood Group ABO System Transferase (ABO) ELISA Kit from MyBioSource.com

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Human Histo-biood Group ABO System Transferase (ABO) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Histo-biood Group ABO System Transferase (also known as ABO) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human ABO antibody. ABO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human ABO Antibody is added and binds to ABO in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABO antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human ABO. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm